ACCELERATED INDUCTION OF HEPATOMAS IN FAST NEUTRON IRRADIATED MICE INJECTED WITH CARBON TETRACHLORIDE
Abstract
Young adult (C57L x A)F(1) hybrid mice received single whole body exposure to fission spectrum fast neutrons (165-306 rad). Subgroups then received single subcutaneous injections of CCl(4), given at 2, 12, 15, or 18 months postirradiation. Control non-irradiated mice received a single injection of CCl(4). Other groups of mice were exposed to single 500 rad dose of 250 KVP X-rays. The incidence of hepatomas increased markedly in neutron-irradiated mice (19%) compared with X-rradiated mice (2%). In neutron-irradiated ice injected with CCl(4), hepatoma incidence attained a value of 61%, three times that in mice irradiated with neutrons only. In addition, marked pleomorphism and atypicality of liver cell nuclei was evdent in almost all neutron-CCl(4) mice, but was observed in only 6 of 47 mice exposed to neutrons only. Of the small group which received CCl(4), but no irradiation, and sacrificed up to 22 months later, none exhibited hepatomas or nuclear abnormalities. CCl(4) seems to act as a promoting agent in liver carcinogenesis. These findings, and other data in the literature, support the concept that hepatoma induction is accelerated by the action of a specific proliferative stimulus on cells bearing a latent radiationinduced alteration.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 19, 1962
- Accession Number
- AD0296242
Entities
People
- L.j. Cole
- P.c. Nowell
Organizations
- Naval Radiological Defense Laboratory