RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD
Abstract
Optimal processing conditions for freezing and thawing whole blood with 7% w/v PVP (Plasdone C) have resulted in reproducible recovery of 97% of the red cells immediately after thawing. Infusion of such preparations in quantities up to one pint have shown an average of 80% of the red cells survive in the circulation for 24 hr. The thawed preparation has 3% of the hemoglobin and 40% of the potassium in the plasma, and it contains a precipitate of %-lipoprotein that is soluble in isotonic saline and plasma. Protein precipitate can be minitized by suspending (1 vol. - 1 vol.) packed red cells in a solution of 14% PVP + 3% human serum albumin before freezing. The same post thaw recovery. 97%, results, but 24-hr. survival averages 88% instead of the 80% for whole blood. Old and young cells lyse to the same extent during freezing and thawing. and both are more susceptible to osmotic lysis after thawing then before freezing.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 20, 1963
- Accession Number
- AD0299116
Entities
People
- A. P. Rinfret