RADIOPROTECTIVE AGENTS: THE EFFECTS OF DEPOLYMERIZED YEAST RIBONUCLEIC ACID ON RADIATED HUMAN CELLS IN TISSUE CULTURE
Abstract
Yeast ribonucleic acid was hydrolyzed with NaOH to produce polynucleotide mixtures which were teeted for radioprotective activity on cultured cell. The depolymerlied RNA stimulated the growth rate of amnion cells during 5-day treatment periods. but had no effect on growth when the cells were treated for a 24-hour period. A 24-hour treatment of amnion cells with the nucleotide mixture prior to 700 r gamma radiation resulted in an increase in the surviving cell populations. This effect appeared to reach a muimum with a polynucleotide concentration of 0.8 mg/ImL The RNA hydrolyzate had no significant effect on radiated KB cells but surviving HeLa and amnion cell populations were increased by 19 and respectively, over controls. This radioprotective response was detected after a minimum of 4 hours of treatment of amnion cells with the test material
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1962
- Accession Number
- AD0401145
Entities
People
- Donald E. Rounds
- William C. Slick