GENETIC ANALYSIS OF MICROORGANISM BY MIXED INFECTION OF ACTIVE PHAGE PARTICLES AND PHAGE OR BACTERIAL DNA
Abstract
Phage a sub 1 cultured by Sg is typically con trolled by this host and variated to be unable to produce plaque on S4S in such good efficiency as when plated on Sg. This restricted phage a sub 1 (Sg) can adsorb on S4S and inject its DNA into the interior of S4S cell. Glucose and man nose can specifically inactivate the purified a sub 1 phage particles. These monosaccharides are also two of four terminal component sugars of salmonella antigen 0(12) which is the receptor for this phage. Methods of extraction and purification of a sub 1 phage DNA have been greatly improved by using the synthetic medium devised for Sg and the refrigerated centrifuge supplied under this contract. With this newly obtained sample the sedimentation rate of a sub 1 phage DNA could be calculated 9.7 x 10 to the -13th power. Mixinfection of this sample of a sub 1 DNA and the active a sub 10s phage particles be longing to the different serological type could also yield some new type phages corresponding to the recombinat, if any, between a sub 1 and a sub 10s, one of which was very different from those previously obtained.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 19, 1963
- Accession Number
- AD0403108
Entities
People
- Isamu Kondo