RADIOPROTECTIVE AGENTS. A CORRELATIVE STUDY OF RNA CONTENT AND THE DEGREE OF RADIORESISTANCE OF CELLS IN VITRO
Abstract
In vitro studies revealed that a 24-hour pre irradiation treatment of an amnion cell line with yeast RNA increased cell survival in proportion to the yeast RNA content in the medium. Conversely, ribonuclease treatment increased radio sensitivity. However, the protective response was observed only after 8 to 10 hours of pre irradiation treatment with yeast RNA. Some in crease in cell survival was recorded at 4 to 6 hours when an alkaline hydrolysate of yeast RNA was used. Microspectrophotometric measurements of cytoplasmic basophilia revealed substantially in creased optical density within 4 hours of incubation with RNA. The delay in a protective response during this period may have been due to the time required for digestion and resynthesis into an active radioprotective agent. A correlation between RNA synthesis and the radio protective response confirmed this hypothesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1963
- Accession Number
- AD0403274
Entities
People
- Donald E. Rounds
- William C. Slick