HOST INFLUENCE ON THE LIPID CONTENT OF VENEZUELAN EQUINE ENCEPHALITIS VIRUS
Abstract
Ada et al reported that the relative proportions of lipid, phospholipid, and cholesterol in the Murray Valley virus propagated in mouse brain (10:0.7:0.9) are different from-ose in seven day normal mouse brain (10:7. 1:1.2). In con trast, the ratio of phospholipid, cholesterol, and triglyceride in influenza virus is similar to that in the host chick embryo chorioallantoic membrane (Frommhagen et al). Analyses of Venezuelan equine encephalitis virus from two host materials (suckling mouse brain and chick embryo), purified by treatment with Celite and protamine, followed by sucrose gradient centrifugation, indicate that the total lipid content of virus from both sources is approximately the same (22 per cent). Moreover, the proportions of lipid, phospholipid, and cholesterol are the same for virus from either host (10:2.7:2. 7), whereas these proportions for four-day normal mouse brain and 11-day normal chick embryo are 10:6.2:3.6 and 10:4.1:3.6, respectively. These results suggest that the arboviruses differ from the myxoviruses in the relationship of viral lipid composition to that of the normal host.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1963
- Accession Number
- AD0407085
Entities
People
- Fred Painter Heydrick
- Ralph Franklin Wachter
Organizations
- United States Army Biological Warfare Laboratories