EFFECTS OF FERRIC NUCLEOTIDES ON MITOCHONDRIAL RESPIRATION

Abstract

Addition of ferric adenosine diphosphates to rat liver mitochondria induced a burst of respiration similar to that given by ADP. The ratio of moles of added Fe(III)ADP to gram-atoms of oxygen removed during the burst was 20% greater than in the corresponding case with ADP. Fe(III)ADP produced the same respiratory stimulation as Mg(II)ADP with malate plus pyruvate, beta- hydroxybutyrate, alpha-ketoglutarate, or proline as substrate. Fe(III)ADP enhanced succinate respiration 60% more than Mg(II)ADP. When mitochondria were treated with succinate and excess ADP, either Fe(III)ATP or Fe(III)oxalacetate stimulated respiration as much as Fe(III)ADP. Several other metal-ADP compounds and EDTA plus ADP also increased succinate oxidation more than Mg(II)ADP. Respiration of mitochondria treated with Fe(III)ADP was inhibited by high concentrations of phenethylbiguanide. Fe(III)ADP reversed the respiratory inhibition caused by low concentrations of phenethylbiguanide.

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Document Details

Document Type
Technical Report
Publication Date
May 20, 1963
Accession Number
AD0407326

Entities

People

  • Charles R. Goucher
  • E. H. Strickland

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  • United States Army Medical Research Laboratory

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  • Biochemistry
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  • Chemical Synthesis
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  • Biology
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