QUANTITATIVE DETERMINATION OF SOME CONSTITUENT LIPIDS IN HUMAN DENTIN

Abstract

A method is described for the extraction of dentin lipids and the separation of these lipids into six components (cholesterol esters, free cholesterol, triglycerides, diglycerides, monoglycerides, and phospholipids). Essentially, the method requires the decalcification of dentin using EDTA and subsequent extraction with diethyl ether, ethanol, and chloroform. The lipid extract is separated into six components by a small silicic acid column and the effluents are quantitated colorimetrically.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1962
Accession Number
AD0407518

Entities

People

  • Kenneth G. Ikels
  • Thomas R. Dirksen

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

Communities of Interest

  • Human Systems
  • Space

DTIC Thesaurus Topics

  • Abstracts
  • Acetic Acid
  • Alcohols
  • Alkanes
  • Blood
  • Blood Serum
  • Cholesterol
  • Chromatography
  • Column Chromatography
  • Fatty Acids
  • Glycerides
  • Government Procurement
  • Lipids
  • Materials
  • Membrane Lipids
  • Methanols
  • Teeth

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Battery Technology and Engineering