GROWTH STUDIES WITH HISTOPLASMA CAPSULATUM
Abstract
In anticipation of studying the infectivity and virulence of the various forms (yeast cells, microconidia and macroconidia, and mycelial fragments) of Histoplasma capsulatum, the effects of selected medium constituents and cultural conditions on growth and viability of the various forms were determined. Mycelial cultures were fragmented by treatment in a blendor to provide small viable units. Hemacytometer counts of such mycelial fragments were compared with viable counts as shown by plating techniques. Although fragmentation increased the number of viable units of mycelial cultures grown in liquid media on the shaker at 27 C, the viable units were only one to five per cent of the hemacytometer counts. Inocula from one-week-old mycelial cultures provided a faster rate of growth in mycelial culturvarious shaken liquid media than did inocula from cultures two to four weeks old.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1963
- Accession Number
- AD0407927
Entities
People
- Carl R. Valentine
- William G. Roessler
- William S. Woodrow
Organizations
- United States Army Biological Warfare Laboratories