GROWTH OF ANIMAL CELLS IN A CHEMICALLY DEFINED SUSPENSION SYSTEM
Abstract
A medium consisting of 13 amino acids, 10 vita mins, 6 inorganic salts, glucose, pyruvate, and methylcellulose was developed for growth of animal tissue cells in suspension. Cultures were grown in 25 milliliters of medium in 100-ml serum bottles in a New Brunswick Gyrotory incubator shaker at 35 C. Populations (4.3 x 10 to the 6th power cells per ml) of cat kidney cells obtained in this medium were comparable with those re ported for the lactalbumin hydrolyzate medium and the insulin-containing chemically defined medium reported earlier from our laboratories. Growth curves were obtained for cat kidney, HeLa, and L cells. Growth of these cells has been maintained continuously for several months by changing the medium on alternate days, and by reducing cell populations to between 5 x 10 to the 5th power and 10 x 10 to the 6th power at the time of medium change. The defined medium is relatively simple in chemical composition, alloys high cell yields, and, combined with the suspension culture method, should be of value in studying various aspect of cell physiology and infectivity.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1963
- Accession Number
- AD0407929
Entities
People
- Stanley C. Nagle
Organizations
- United States Army Biological Warfare Laboratories