STUDY ON THE INTRACELLULAR CALCIFICATION BY ORAL LEPTOTRICHIA
Abstract
The intracellular calcification of oral filamentous micro-organisms which were identified Bacterionema matruchotti from the bacteriological examinations in this study was analysed chemically in relation to its mechanism. The intracellular calcium and phosphorus were found to increase following the in vitro calcification. In addition, the ratio of calcium and phosphorus approximates to 2.12 which is the ratio of themselves in hydroxyapatite. These findings were observed in both living and killed cells; therefore, it can be said the intracellular calcification is independent from the metabolic process of the microorganisms. Heating or magnesium ion which possessed the specific inhibition against the in vitro calcification of rachitic cartilage was found to affect the intracellular calcification as well. From the experiment on the other inhibitory factors, formalin, if it exists in the calcification throughout, was found most effective. These findings suggest that the intracel lular calcification consisted of three phases: crystal nucleation, crystal growth, and crystal limitation as the phenomena observed in other organic tissues. Also, the existence of intra cellular organic substance responsible to the calcification, as collagen found in bone tis sues, was assumed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 30, 1963
- Accession Number
- AD0409937
Entities
People
- Waichi Yonezawa
Organizations
- Tokyo Dental College