A MOBILE LABORATORY UNIT FOR EXPOSURE OF ANIMALS AND HUMAN VOLUNTEERS TO BACTERIAL AND VIRAL AEROSOLS
Abstract
A mobile laboratory exposure unit designed for inoculating human volunteers with bacterial and viral aerosols is described. Included are methods of generating aerosols of both small and large particles and of determining the size and concentration of particles. The aerosols contain one to 8,000 microorganisms per liter of air. Evaluation of the equipment includes tests for safety of personnel, determination of spray factors for predicting concentration of suspensions, comparison of samplers for more precise measurement of dilute aerosols (one organism per liter), comparisons between organism concentrations at sampling points and exposure ports, and between animal virulence assays. Human volunteer exposures include (a) small-particle (one- micron diameter)AEROSOLS DISSEMINATED BY A Collison atomizer; (b) large-particle (8- to 10.5-micron diameter) aerosols generated by a vibrating reed device; (c) respiratory vaccination with living vaccine cells of Pasteurella tularensis; and (d) small-particle aerosols containing Coxsackie A21 (Coe agent). Results indicate the equipment is capable of generating aerosols that can be meas ured with a high degree of confidence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1963
- Accession Number
- AD0410156
Entities
People
- William Robert Griffith
Organizations
- United States Army Biological Warfare Laboratories