METHOD OF PROCESSING BACTERIOLOGICAL PREPARATIONS WITH FLUORESCENT ANTIBODIES,

Abstract

The work deals with preparation, fixation and staining of bacteriological preparations with fluorescent antibodies (direct and indirect methods). The authors' investigations have de monstrat that the temperature regimen and duration of smear staining by means of fluores cent antibodies are important factors, condition ing the intensity and specificity of microbial cells fluorescence. The composition of the washing liquids and the methods of fixation of the preparations had no essential effect on the capacity of bacterial cells to be stained with specific fluorescent antibodies. (Author)

Document Details

Document Type
Technical Report
Publication Date
May 24, 1963
Accession Number
AD0410695

Entities

People

  • I. O. Dashkevich
  • S. I. Dyakov

Organizations

  • Joint Publications Research Service

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biological Staining And Labeling
  • Fluorescence
  • Intensity

Fields of Study

  • Biology

Readers

  • Immunology
  • Systems Analysis and Design

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech