EVIDENCE FOR A NEW METHYLATED SUGAR: 3-0 METHYL MANNOSE IN THE EXTRACELLULAR POLY SACCHARIDE OF COCCIDIOIDES IMMITIS
Abstract
Large amounts of polysaccharide were obtained from the culture filtrate of the pathogenic fungus Coccidioides immitis grown on a glucose, ammonium lactate, inorganic salts medium. The polysaccharide was precipitated with ethanol, hydrolyzed, and analyzed qualitatively and quantitatively for onosaccharide constituents. Evidence is presented for the identification of the sugars as galactose, glucose, mannose, and 3-0-methyl mannose. 3-0-Methyl mannose has not previously been found in any living organism or as a natural product. The identification of 3-0-methyl manose is based on the following evidence: demethylation yielded mannose; methoxyl analysis indicated a monomethyl sugar; periodate oxidation at acid pH and 0C yielded a product that has the chromatographic properties of 2-0 methyl arabinose; and the periodate oxidation product was converted to arabinose by demethylation. Qualitative analysis of the sugar components of the polysaccharide fractions precipitated by increasing amounts of ethanol indicate that there are at least three different polysaccharides in the culture filtrate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1963
- Accession Number
- AD0413350
Entities
People
- Eugene P. Goldschmidt
- Gordon W. Taylor
Organizations
- United States Army Biological Warfare Laboratories