ENCHANCEMENT OF STAINING INTENSITY IN THE FLUORESCENT ANTIBODY REACTION

Abstract

A method was developed for enhancing the staining intensity of bacterial smears treated with fluorescent antibody. The procedure involved the substitution of carbonate-bicarbonate buffer pH 9.0 for phosphate-buffered saline pH 7.2 in the washing of slide preparations and in buffering the glycerol mounting medium. A marked in crease in fluorescent brightness was obtained with all dilutions of homologous conjugate employed with three test strains (Bacillus anthracis, Brucella abortus, and Serratia marcescens).

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1963
Accession Number
AD0413362

Entities

People

  • Abe Pital
  • Sheldon L. Janowitz

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Antibodies
  • Biological Laboratories
  • Biological Staining And Labeling
  • Dilution
  • Efficiency
  • Fluorescence
  • Government Procurement
  • Immune Serums
  • Intensity
  • Luminescence
  • Materials
  • Methanols
  • New York
  • Proteins
  • Quantum Efficiency
  • United States

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology
  • Microbial Pathology