HYPOTHALAMIC SECRETORY FACTOR FOR ADRENOCORTICOTROPIC HORMONE (SF-ACTH).

Abstract

Research on the biological assay and purification of the hypothalamic secretory factor for ACTH (HSF-ACTH) has been described. Extracts of hypothalamic tissue are evaluated by testing for decrease in adrenal ascorbic acid (as an indicator of endogenous or exogenous ACTH) in three types of rats. Rats anesthetized with pentobarbital are used to estimate proper dose levels and to detect nonspecific stressful contaminants. Rats anesthetized with pentobarbital and treated with morphine, which do not respond to moderate stimulation by nonspecific substances, are used to estimate the amount of HSF-ACTH, but they also respond to ACTH. Hypophysectomized rats are used to estimate the amount of exogenous ACTH that may contaminate the HSF-ACTH preparations. (Author)

Document Details

Document Type
Technical Report
Publication Date
Jan 16, 1963
Accession Number
AD0415479

Entities

People

  • Paul L. Munson

Organizations

  • Harvard School of Dental Medicine

Tags

DTIC Thesaurus Topics

  • Assays
  • Bioassay
  • Environmental Pollutants
  • Hormones
  • Indicators
  • Morphine
  • Pituitary And Hypothalamic Hormones And Analogues
  • Vitamin C

Fields of Study

  • Medicine

Readers

  • Analytical Chemistry
  • Cardiovascular Physiology