ENZYMATICALLY AND PHYSICALLY INDUCED INHERITANCE CHANGES IN BACILLUS SUBTILIS

Abstract

When a culture of Bacillus subtilis strain 168 is treated with a solution of crystalline lysozyme, the bacteria pass through an osmotically sensitive bacillary phase to the protoplast stage. All of the protoplasts can be recovered as L- colony-forming elements on complex or chemically defined osmotically stabilized agar media. Protoplasts or L-bodies can be propagated in the L-state indefinitely or can be mass-reverted to the bacillary state by altering medium in gredients.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1963
Accession Number
AD0416643

Entities

People

  • Otto E. Landman
  • Sidney Halle

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Anti-Bacterial Agents
  • Bacteria
  • Bacteriology
  • Bacteriophages
  • Cell Division
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Fungi
  • Genetics
  • Gram-Negative Bacteria
  • Microbial Genetics
  • Microbiology
  • Microorganisms

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