RAPID, ACCURATE ANALYSIS OF BLOOD LACTATE

Abstract

A method is described for the enzymatic analysis of blood L-lactic acid. The method is based on existing methods, but incorporates certain unique features which permit a rapid and precise analysis of a large number of samples. One of the more important features is the use of a simplified procedure for correcting the problematic drift of the unstable reaction mixture. In addition, conditions for the enzymatic activity were established which facilitate complete oxidation of lactate in 30 to 35 minutes. A comparative analysis of several blood filtrates, using the present method versus a well-known chemical method, as well as recovery analyses using the two methods, establishes the validity of the present test system.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1963
Accession Number
AD0418252

Entities

People

  • Edgar W. Williams
  • James P. Ellis Jr.
  • Stephen M Cain

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

Communities of Interest

  • Space

DTIC Thesaurus Topics

  • Absorption
  • Accuracy
  • Acids
  • Aerospace Medicine
  • Air Force
  • Calibration
  • Government Procurement
  • Governments
  • Hydrazines
  • Incubation
  • Instructions
  • Intervals
  • Lactic Acid
  • Mixtures
  • Physiology
  • Sodium Hydroxide
  • Standards

Fields of Study

  • Biology
  • Engineering

Readers

  • Analytical Chemistry
  • Computational Modeling and Simulation