DEVELOPMENT OF EXPENDABLE LINER CENTRIFUGE AND SOLUTION FLOW CONTRO SYSTEM FOR GLYCEROLIZATION AND DEGLYCEROLIZATION OF RED BLOOD CELLS.
Abstract
The object of this work was to learn how to design a practical system for long-term preser vation of blood using a process consisting of separation of plasma from red cells, glyceroli zation of red cells, storage of plasma and glycerolized red cells separately at -80 C until needed, thawing, deglycerolizing the red cells and resuspending them. This process has been under clinical tria for several years using manually operated research equipment, and it is desired to reduce greatly the amount and level of technician skill needed to use the process on a large scale. Development of a system which in corporated use of an expendable centrifuge liner, a glycerolization and deglycerolization kit, and automation of the glycerolization and deglycero lization cycles, was the approach used to mini mize use of skilled operating technician atten tion. The performance of the new system was brought to a level at which the biochemical assays compared very favorably with results ob tained with the equipment and procedures used on clinical trial during recent years. Operational feasibility of the system was demonstrated and the practicability of achieving minimal de pendence on skilled operating technicians appears to be well within reach. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1963
- Accession Number
- AD0418358