INDUCTION OF HOMOGRAFT TOLERANCE IN SUBLETHALLY X-IRRADIATED ADULT MICE,
Abstract
An attempt has been made to achieve homograft tolerance in sublethally irradiated adult mice under conditions which avoid secondary disease. Allogeneic mice which share the same H-2 locus, but differ at other histocompatibility loci, have now been used with some success. A tolerant state has been produced in CBA (H-2k) mice by first exposing them to sublethal X-radiation (500 rad) or urethan-treatment coupled with irradiation, and then injecting C3H (H-2k) spleen (18 x 10 to the 6th power) and/or bone marrow (42 x 10 to the 6th power) cells within 3 days post irradiation. These mice have retained subsequent C3H skin homografts (over 165 days) but have rejected BALB/c skin grafts (H-2d) within 33 days. On the other hand, irradiated and urethan-treated controls, uninjected or injected with X-ray inactivated (2000 rad in vitro) C3H cells, rejected both C3H and BALB/c homografts by 70 days. Similar results, although less definitive, have been obtained using C3H (Hd2k) or BALB/c (H-2d) as the recipient mice and CBA (H-2k) or DBA/2 (H-2d) mice respectively, for the donor cells. Such procedures, however, have been unsuccessful in producing homograft tolerance between donor and host F1 hybrid mice which have one H-2 histocompatibility locus in common but differ at the other. The significance of these results is discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 19, 1963
- Accession Number
- AD0419563
Entities
People
- Leonard J. Cole
- W. E. Davis
Organizations
- Naval Radiological Defense Laboratory