PATHOGENESIS OF EXPERIMENTAL TULAREMIA IN MONKEYS: EFFECT OF PARTICLE SIZE

Abstract

The purpose of the experiments described in this report was to determine the primary sites of infection and subsequent histogenesis of lesions in lungs of rhesus monkeys exposed to the SCHU S4 strain of Pasteurella tularensis in aerosols consisting of particles either one or eight microns in diameter. The monkeys were sacrificed at 1/3, 12, 24, 72, and 96 hours after exposure and tissues were examined by conventional pathological techniques and fluorescent antibody methods. Under the conditions of these experiments the respiratory bronchiole was the primary site of infection in the lung of rhesus monkeys. Intracellular P. tularensis was demonstrated in respiratory bronchioles by fluorescent antibody straining of tissues obtained 20 minutes after exposure to aerosols of one-micron particles.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1963
Accession Number
AD0422365

Entities

People

  • Charles W. Beard
  • Edward B. Derrenbacher
  • James R. Rooney
  • John D. White
  • Patricia A. Prickett

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Bacteria
  • Biomedical Research
  • Cells
  • Health Services
  • Infection
  • Laboratory Animals
  • Lymphatic System
  • Materials
  • Microorganisms
  • Particle Size
  • Pathogenesis
  • Public Health
  • Rhesus Monkeys
  • Rodents
  • Tissues

Fields of Study

  • Biology

Readers

  • Aerosol Science/Aerosol Physics
  • Cardiovascular Physiology
  • Immunology