SPECIFIC SUPPRESSION OF WOUND HEALING IN MICE BY GRAFT-VS-HOST REACTION,
Abstract
Young adult LAF-1 mice bearing full-thickness skin grafts from A/HeJ mice received a single dose of 500 rad X-radiation, followed by ip injection of 6 x 10 to 6th power lymph node cells from normal A/HeJ donors; controls were injected with inactivated A/HeJ lymph node cells. Circular punch skin wounds through the donor skin graft and adjacent host skin were made 8 days later. The mice receiving the intact parental strain lymph node cells showed symptoms of transplantation disease, while the controls were free of such signs. Skin wound healing was assessed histologically in mice sacrificed 3 and 6 days after wounding, permitting a comparison of healing in the donor skin-versus-host skin on the same mouse. The findings indicate a definite specific inhibition of wound healing in LAF-1 skin compared to A-skin in the mice suffering from transplantation disease. In each of 7 mice examined 6 days after injection of A-strain lymphoid cells, healing in the grafted A-strain skin was definitely greater than in the host's own LAF-1 skin. Thus, the LAF-1 skin at this time showed only minimal to early healing, while the wounds in the grafted A-skin were completely closed in 4 of 7 cases, of which 2 were healed. The possible theoretical basis for the specific inhibition of wound healing in graft-versus-host disease is briefly discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 14, 1963
- Accession Number
- AD0425518
Entities
People
- Leonard J. Cole
- P. C. Nowell
- W. E. Davis Jr.
Organizations
- Naval Radiological Defense Laboratory