RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD. THE ANALYTICAL DETERMINATION OF POLYVINYLPYRROLIDONE,
Abstract
Two methods have been developed for the analytical determination of PVP in serum and urine from experimental dogs. In the iodine titration method, the sample is treated with an iodine solution to form a PVP-I2 complex which is spearated from the solution by centifugation, and then disolved in methyl cellosolve. The iodine is titrated with standard thiosulfate solution. In the second method, the absorption of infrared radiation by PVP is measured at a wavelength of 7.75 microns. The iodine method gives values for PVP concentrations in serum and urine which are accurate within = 10%, and the infrared method gives similar accuracy for PVP in serum. In the case of urine samples analyzed by the infrared method, creatinine is sometimes administered to the experimental dogs along with PVP. When the creatinine/PVP weight ratio is less than about 1/1, the accuracy of the PVP determinations by the infrared method is within about = 10%, but when the creatinine/PVP ratio exceeds about 1/1, the accuracy varies from = 10% to = 50% as the creatinine/PVP ratio varies from about 1 to about 100. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 13, 1963
- Accession Number
- AD0425883
Entities
People
- A. P. Rinfret