RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD. XVI. PROCESS OPTIMIZATION.
Abstract
In process I plasma is separated from red cells by conventional centrifugation and the cells re suspended in an additive solution containing polyvinylpyrrolidone (PVP). It has been found that, with a small quantity of albumin in the ad ditive, PVP concentrations reqquired for red blood cell protection can be decreased and the in-vitro recoveries significantly improved. PVP alone is less efficient in providing protection against freeze-thaw damage in this type of preparation. In Process III blood is collected directly into an additive-anticoagulant mixture or collected into anticoagulant and subsequently mixed with the protective additive. This is the system of choice only when prefeeze handling is to be kept at a minimum. Recoveries% can be at tained with this process using Plasdone-C (PVP with an average M.W. of 40,000). Lower molecular weight PVPs have provided equivalent protection of cells from freeze-thaw hemolysis, however, these cells are less stable osmotically. Pre liminary studies of the prefreeze removal of precipitate from the interaction of PVP and plas ma in Process III indicate that red cell recovery is not affected by the procedures involved. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 13, 1963
- Accession Number
- AD0426893
Entities
People
- A.p. Rinfret