FLUOROMETRIC ANALYSIS OF AMIDASE AND ALKALINE PHOSPHATASE IN NEONATAL RAT THYMOCYTES: PEAK ACTIVITIES AT TWO DAYS OF AGE,
Abstract
Activities of four amidases and alkaline phosphatase were studied in lymphoid cells obtained from the thymuses of rats during the first 21 days of post natal life. Alkaline phosphatase activity increases rapidly from a value of 1.4 on day one to 24.4 on day 2, and declines steadily until day 7 from which time there is a constant activity (3.5) through day 21. Similar activity profiles obtain for leucyl, alanyl and phenylalanyl amidases showing maxima on day 2 of 26.2, 28.9, and 21.3 respectively. Comparatively constant activity levels are reached after one week, leucyl having the lowest value, 3.7, phenylalanyl slightly higher, 4.5, and alanyl the highest, 9.3. Glycyl amidase activity is nil on day one, rises to a maximum activity of 12.4 on day 2, and falls off again to an immeasurable quantity by day 14. Histological examination of thymuses over the period studied revealed a relative increase in the number of large lymphoid cells. The changes in enzyme activities appear to reflect changes in the large thymocyte population. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 18, 1963
- Accession Number
- AD0427290
Entities
People
- L. J. Greenberg
- Leonard J. Cole
Organizations
- Naval Radiological Defense Laboratory