THE AGAR/GEL PRECIPTIN TECHNIQUE IN ANTHRAX ANTIBODY DETERMINATIONS

Abstract

A modification of the agar/gel precipitin inhibition technique of Thorne and Belton for detecting anthrax antibodies reduces inconsistency of visually determined end points on the same sera observed by different technicians. Determination of the minimum reacting concentrations of the anthrax antigen and antibody reagents, modifications of the visualization apparatus, methods for combining reagents, and length of incubation periods contributes to the ease of the end point determinations and the uniformity of results. When compared with the previous technique, the modified procedure is less time- consuming and retains satisfactory reproducibility, simplicity, specificity, and sensitivity.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1963
Accession Number
AD0427660

Entities

People

  • John G. Ray Jr.
  • Paul J. Kadull

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Biomedical Research
  • Filter Paper
  • Filters
  • Filtration
  • Food Poisoning
  • Gram-Negative Bacterial Infections
  • Immune Serums
  • Immunity
  • Immunomodulation
  • Laboratory Animals
  • Materials
  • Precipitation
  • Production
  • Rodents
  • Sodium Compounds

Readers

  • Immunology
  • Systems Analysis and Design