OBSERVATIONS OF A PHENOTYPIC MODIFICATION OF VARIOLA VIRUS IN TISSUE CULTURE
Abstract
An analysis was made to define the steps in the growth cycle of variola virus where the qualitative difference between cell-associated virus and supernatant virus is reflected. Thus far, results show that adsorption and/or penetration is greater in extent and occurs more rapidly with cell-associated virus than with supernatant virus. Further, cell-associated virus has a shorter lag phase, and is released into the medium more rapidly and to a significantly higher titer than supernatant virus. It is concluded that supernatant virus is because relatively little is released for infection of new cells, and what is released is of relatively poor infectious quality. The same observation was made with vaccinia virus, but the change from p+ to p- was more gradual.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1963
- Accession Number
- AD0428033
Entities
People
- Arthur Brown
- Elliott H. Purlson
Organizations
- United States Army Biological Warfare Laboratories