RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD. XVII. FREEZE THAW DAMAGE AS A FUNCTION OF CELL AGE USING FE SUB 59 LABELED HUMAN AND RABBIT ERYTHROCYTES.
Abstract
The relationship between in vivo erythrocyte age and susceptibility to freeze-thaw damage was studied using radioactive iron-59. This isotope is used to label selectively the young erythrocyte population as the cells are being released into circulation by the bone marrow. These studies, carried out with both human and rabbit blood, were designed to determine whether hemolysis occurring as a result of freeze-thaw damage is greater in the older cells which are destined to be removed from circulation or smaller in the younger erythrocytes. The results show that young erythrocytes are in general more resistant to freeze-thaw injury than are the older segments of the population, indicating that there is a definite relation between freeze-thaw damage and in vivo cell age. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 16, 1964
- Accession Number
- AD0428211
Entities
People
- A. P. Rinfret