RESEARCH, DEVELOPMENT AND FARBRICATION OF AN EXPENDABLE BIOELECTROCHEMICAL DETECTOR SYSTEM
Abstract
This report describes the use of open cell urethane foam pads for supporting the starch immobilized enzyme. Eight per cent starch gel is prepared and enzyme added as the gel cools. Subsequently, foam pads are saturated with the gel, the excess gel squeezed out, and the pads freeze-dried. Experiments show that pads prepared in this manner and exposed to a stream of 1 ml. of substrate and 1-1/2 liters of air per minute will continue to hydrolyze substrate in excess of 2 hr. This report also describes the operation of an experimental detector for a period of 2 hr. and the response of the detector to an enzyme inhibitor. This test employed perforated grid electrodes fabricated from platinum sheet. These electrodes were in contact with the surfaces of the foam pad. The feasibility of bioelectrochemical monitoring of enzyme activity as a method for detecting nerve agents is demonstrated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 19, 1964
- Accession Number
- AD0430254
Entities
People
- Elven K. Bauman
Organizations
- MRIGlobal