CELLULAR OXYGEN CONSUMPTION AT LOW OXYGEN TENSIONS,
Abstract
As part of a preliminary investigation into certain aspects of exobiology, a baseline study has been made of various factors including pH, cell concentration, temperature, and metabolic state which might affect the critical oxygen tension (C. O. T.) of Saccharomyces cerevisiae. The metabolic state and temperature are found to have a significant effect on the C.O.T. The C.O.T. in the starved condition is independent of temperature, while the exogenous state exhibits an increase in C. O.T. with an increase in temperture. A comparison of the C.O.T. value at 25 C. gave approximately 4mm. Hg Po2 in the exogenous state and less than l mm. Hg Po2 in the starved state. These results, in general, confirm earlier studies. No effect of the C.O.T. by pH or cell concentration changes could be found. The C. O. T. of cytochrome-deficient Neurospora crassa mutants has been compared with that of normal strain and no difference was observed. The polarographic methodology as applied to cellular oxygen consumption is described, and the practical significance that the C. O. T. measurement may have in study of cellular processes is discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1963
- Accession Number
- AD0430363
Entities
People
- J. Ryan Neville
- Robert J. Reyes
Organizations
- United States Air Force School of Aerospace Medicine