ELECTROPHORETIC AND PHOSPHATE ANALYSES OF HEMOLYSATES OF STORED BLOOD.
Abstract
Increases in pH or in ionic strength of the cacodylate buffer are responsible for a decrease in the concentration of a slow-moving boundary (Component B) in the electrophoretic pattern of human erythrocyte hemolysates. The patterns for crystalline hemoglobin, aged blood and phosphatase-treated hemolysates, all of which contain trace amounts of organic phosphate and Component B, are almost identical. The addition of ATP, GTP, ITP or 2,3-DPG to crystalline hemoglobin or hemolysates of aged blood increases Component B concentration markedly, while ADP, GDP or IDP cause only moderate increases, and the mononucleotides have practically no effect. The organic phosphate concentrations of erythrocytes of ACD and CPD bloods, supplemented on 0 day with varying amounts of inosine, adenosine, adenine or mixtures of these compounds, show marked differences during anaerobic storage. Organic phosphate concentrations are maintained at the highest levels in CPD bloods supplemented with adenosine, adenosine + inosine, and adenosine + adenine. Statistical analysis shows a high degree of correlation between organic phosphate and Component B concentrations, but the slopes of the regression lines vary considerably. Both Component B and organic phosphate concentrations are maintained at appreciably higher levels during aerobic than during anaerobic storage of bloods. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 31, 1966
- Accession Number
- AD0430615
Entities
People
- Alfred Chanutin
Organizations
- University of Virginia