CALCULATED NATURAL FREQUENCIES, NORMAL MODES, AND FORCED VIBRATIONS OF USS KATE (SSN 578)
Abstract
Ricin, the highly toxic hemagglutinating protein, was isolated from the seeds of Ricinus Communis L. The defatted castor meal firstly extracted with 10% sodium chloride solution, followed by fractional precipitation with ammonium sulfate, the highly toxic ricin fractions I and II were obtained. Recin I was the most toxic fraction, killing the mouse of 20 Gm. at a dose of 1 mcg. by intraperitoneal injection of 4 days. The qualitative determination of Ricin fractions was by paper chromatographic method. Digestion of Ricin I by pepsin and trypsin was found to take place slowly in 3 days. A prolonged enzymatic digestion was made for 2 weeks, it broke down about 39-48% of low molecular products. Antitoxic serum to Ricin I was prepared by immunization of rabbits with an increasing amount of formalinized toxoid (formalin treated Ricin I). The concentration of 0.5-5% formalin were used resulting about a 100-1000 fold reduction in toxicity of Ricin I. Marked loss of antifenicity occured with formalin at pH 8.5 and above.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1982
- Accession Number
- AD0430720
Entities
People
- R. M. Murray Jr
Organizations
- National Taiwan University