THE LIPIDS OF SARCINA LUTEA. 1. FATTY ACID COMPOSITION OF THE EXTRACTABLE LIPIDS
Abstract
The extractable lipids of Sarcina lutea were separated into several fractions by a combination of column and thin-layer chromatography. Qualitative and quantitative characterization of the fatty acid content of these lipid fractions was accomplished by means of gas-liquid chromatography and infrared analysis. Of the total extract, the lipids consisted of 2.1% free fatty acids, 51.0% glycerides, and 22.7% complex lipids; they had a fatty acid content with a complete spectrum of carbon numbers from C8 to C22. The fatty acids included a large component of branched-acids in addition to the normal straight chain acids. The branched-acids, comprising 40% of the fatty acids analyzed, constituted a homologous series of iso acids from C12 to C19. Two 18-carbon unsaturates were found, cis-9-octadecenoate and cis-11-octadecenoate. A relatively high percentage (20.5%) of the extractable material from S. lutea was found to be hydrocarbon. This material was not further characterized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1964
- Accession Number
- AD0430783
Entities
People
- Charles K. Huston
- Phillip W. Albro
Organizations
- United States Army Biological Warfare Laboratories