ACETATE, FORMATE AND RELATED ENZYMES AS FACTORS IN METABOLISM.
Abstract
Homogenates of the larvae of Phormia regina oxidize formate to carbon dioxide. The formate oxidizing enzyme was purified about eighteen-fold, and simultaneously a similar purification of catalase was obtained. Formate oxidation is competitively inhibited by nitrite and ethanol and is oxidized by a catalase-peroxide complex. Hydrogen peroxide is generated by oxidation of reduced sulfhydryls like glutathione and homocysteine. It is also produced by the oxidation of hypoxanthine by xanthine oxidase, NADH sub 2 and NADPH sub 2 plus plus through flavins, and possibly folate. Thus formate oxidation in Phormia involves coupled reactions between enzyme systems producing hydrogen peroxide (except glutathione oxidation if it is a non-enzymatic reaction) to form a complex with catalase, which in turn oxidizes formate to carbon dioxide. If Phormia regina is grown on a diet in which carnitine replaces choline, then a new phosphatid, phosphatidyl methylcholine is made. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 31, 1962
- Accession Number
- AD0431955
Entities
People
- Robert W. Newburgh
Organizations
- Oregon State University