PURIFICATION OF HISTAMINE SENSITIZING FACTOR OF BORDETELLA PERTUSSIS.
Abstract
Histamine Sensitizing Factor (HSF) of Bordetella pertussis was studied. To improve the cultivation method for HSF preparation, growth conditions of B. pertussis were investigated. Ribonucleotides as well as spermine stimulated the growth in a semi-synthetic medium. A new medium suitable for maintenance and surface bulk culture was devised. Intracellular localization of HSF and lethal toxin were studied. HSF was mainly localized in the cell wall prepared by Mickle disintegration, and toxin was found in protoplasm Ribosomes prepared from sonicate contained considerable amount of HSF and toxin. Properties and biological activities of HSF preparation were investigated. Homogeneous preparation of HSF has not yet been obtained. Protective antigen potency against intracellular challenge with B.pertussis was observed in HSF preparation, but conclusion is reserved for further investigation. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 1963
- Accession Number
- AD0432175
Entities
People
- Yoshio Kuwajima
Organizations
- Osaka City University