PRESENCE OF DONOR SPECIFIC GAMMA-GLOBULINS IN SERA OF ALLOGENEIC MOUSE RADIATION CHIMERAS,
Abstract
Young adult DBA/2 mice received a single whole body X-ray exposure of 840 rad (an otherwise lethal dose) followed by an intravenous injection of 8 x 10 to the 6th bone marrow cells from normal (C3H x DBA/2)F1 donors. Control groups included syngeneic (isogenic) chimeras, i.e., DBA/2 mice which received 840 rad plus an injection of 8 x 10 to the 6th DBA/2 marrow cells. Blood serum from these chimeras was collected at times up to 9 months postirradiation, and analysed for total gamma-globulin levels and for specific donor or host type gamma-globulin isoantigens. Marrow-donor type gamma-globulins (Igal) were found in the serum of the allogeneic chimeras at various times up to 9 months after irradiation. By contrast, the sera from the syngeneic chimeras were negative for Igal gammaglobulin, and showed high levels of Iga3 gammaglobulin, the DBA/2 type. It was shown also that the allogeneic chimeras were specifically tolerant to appropriate skin homografts; whereas, the syngeneic chimeras rejected both A-strain skin grafts and (C3H x DBA/2)F1 skin grafts. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 02, 1964
- Accession Number
- AD0432416
Entities
People
- L. A. Herzenberg
- Leonard J. Cole
Organizations
- Naval Radiological Defense Laboratory