CONDITIONS NECESSARY FOR TRANSFORMATION TO PROTOTROPHY AND TO THE ABILITY TO SYNTHESIZE POLYGLUTAMIC ACID IN BACILLUS LICHENIFORMIS

Abstract

This report presents studies on the growth conditions necessary for transformation to prototrophy of 14 auxotrophs of B. licheniformis. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimum growth conditions for a serine-deficient mutant, transformation frequencies of 0.1% were obtained. A defined medium for tube transformation of competent cells is described. In addition, this report presents evidence for the transformation of three non-encapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1964
Accession Number
AD0432424

Entities

People

  • C. G. Leonard
  • D. K. Mattheis
  • M. J. Mattheis
  • R. D. Housewright

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Amino Acids Peptides And Proteins
  • Biological Laboratories
  • Cells
  • Culture Techniques
  • Deoxyribonucleic Acids
  • Frequency
  • Glycerols
  • Incubation
  • Materials
  • Microorganisms
  • Neutral Amino Acids
  • Nucleic Acids
  • Peptides
  • Sugar Alcohols
  • United States

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics