PURIFICATION AND LIPID CONTENT OF VEE VIRUS
Abstract
The virus of Venezuelan equine encephalitis (VEE) was purified from chick embryo suspensions by two methods, and the total lipid content and RNA content of the purified virus preparations were compared. The lipid content of VEE virus purified by sucrose gradient centrifugration was much lower (24 per cent) than that of VEE virus purified without gradient centrifugation (54%). Virus of lower lipid was still fully infectious. With the decrease in lipid content of the virus there was a concomitant increase in RNA content. Electron microscopic examination of purified VEE irus showed that the virus had an average diameter of 68 millimicrons and possessed a center core about 30 millimicrons in diameter.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1964
- Accession Number
- AD0432471
Entities
People
- Emil W. Johnson
- Fred P. Heydrick
- Ralph F. Wachter
Organizations
- United States Army Biological Warfare Laboratories