THE CHEMICAL MODIFICATION OF NECROGENIC AND PROTEOLYTIC ACTIVITIES OF CROTALIDAE VENOM AND THE USE OF EDTA TO PRODUCE A VENOM TOXOID
Abstract
This report represents an investigation of the proteolytic activity, necrolytic activity, and antigenicity of untreated venom and venom which had reacted with ethylenediaminetetraacetic acid (EDTA). Agkistrodon piscivorus venom treated with EDTA lost ability to digest casein. Neither sodium phosphate nor sodium bicarbonate restored this ability. The hemorrhagic and necrotizing activity of this venom was essentially absent following EDTA treatment. EDTA treated venom which did not produce necrosis and hemorrhage stimulated the production of antibodies which neutralized the local and systemic toxic activity of untreated venom. The usefulness of EDTA in the treatment of snakebite should be investigated and attempts should be made to isolate and characterize the necrogenic and proteolytic components of snake venom.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 09, 1963
- Accession Number
- AD0433665
Entities
People
- Charles R. Goucher
- Herschel H. Flowers
Organizations
- United States Army Medical Research Laboratory