GENETIC, SEROLOGIC, AND BIOCHEMICAL STUDIES ON VIRAL INFECTION AND LYSOGENIZATION.
Abstract
Salmonella phage undergoes a two-way host-controlled viriation as described before. Besides multiplicity activation (MA), a thermo-labile factor responsible for phage growth restriction was found. Experiments with P32-labled phages show that the DNA of the restricted phage is degraded rapidly after injection of the nonpermissive host, and that the degradation is prevented by heating the cells prior to infection. MA-responsible, thermolabile, and DNA-destroying factors are suggested to be identical. Chemical, serological, and genetic studies have revealed the followings: Salmonella senftenberg 87Aa' with O antigen 3 alone has alpha-galactosyl-mannosylrhamnose as serological determinant; specificity 3 is determined by mannosyl-rhamnose; another cross reaction between El strains and 87Aa' is attributed to alpha-galactosyl structure; 87Aa' is suspected to carry defective prophage lacking conversion genes. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 19, 1964
- Accession Number
- AD0436554
Entities
People
- Hisao Uetake