RIFT VALLEY FEVER VIRUS ASSAY IN SERUM-FREE HAMSTER KIDNEY MONOLAYERS
Abstract
High titers of some arboviruses, including Rift Valley fever (RVF) virus, are obtained in cell cultures without any observable cytopathogenic changes. In the absence of serum, however, a rapid destruction of tissue-culture cells occurred with RVF virus. Preparation of virus dilutions in a serum-free maintenance medium and observation of infected tube monolayers has been found to be an effective method for achieving reproducible titrations of RVF virus. It is possible to calculate a median cytopathogenic effect dose (CPED50) comparable to titers determined by a plaque technique but more sensitive than in vivo titration. The tube monolayer titration is more economical, more rapid, and simpler to perform than previously used methods.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1964
- Accession Number
- AD0437967
Entities
People
- John J. Boyle
Organizations
- United States Army Biological Warfare Laboratories