STUDIES ON THE PURIFICATION OF VACCINIA VIRUS

Abstract

Various methods are described and compared for removing extraneous matter from vaccinia virus suspensions. Of ten procedures tested, differential centrifugation and Freon extraction were the most successful. Both the partially purified suspensions and the dried products prepared from them had high virus titers in embryonated eggs and high infectivity for mice. Although both processes resulted in products with the same titers, the differentially centrifuged product was approximately tenfold more purified as shown by the percentage solids of the suspensions before freeze drying.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1964
Accession Number
AD0442409

Entities

People

  • Jean M. Riley
  • Michael D. Orlando
  • William C. Patrick Iii.

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Adaptive Immunity
  • Animals
  • Biological Laboratories
  • Drying
  • Efficiency
  • Electron Microscopy
  • Extraction
  • Freeze Drying
  • Immunity
  • Inoculation
  • Laboratory Animals
  • Materials
  • New York
  • Particles
  • Poxviridae Infections
  • Vaccines
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Mathematics or Statistics