HOST INFLUENCE ON PLAQUE FORMATION BY VENEZUELAN EQUINE ENCEPHALITIS VIRUS
Abstract
In a study of the influence of the host on characteristics of Venezuelan equine encephalitis (VEE) virus, it was observed that virus populations propagated in the chick embryo, mouse brain, or monolayers of L cells differ in the following characteristics depending upon the host: (a) banding patterns in sucrose gradients, (b) ratio of soluble to insoluble lipids (in petroleum ether), and (c) size of plaques formed by both crude and purified virus when assayed in chick fibroblast monolayers. This report deals mainly with differences in plaque formation. The origin of the inoculum (chick embryo, mouse brain, or tissue culture) and the host (chick embryo, mouse brain, or tissue culture) both contributed to the determination of the ratio of large to small plaques obtained VEE virus seeds prepared in each of the three hosts and used throughout this investigation were derived from the same equine strain. When chick embryo seed was used as inoculum, only large-plaque-forming virus was obtained from all three hosts. On the other hand, when mouse brain or tissue culture seeds were used as inocula, both large- and small-plaqueforming virus was obtained from all three hosts, with the plaque ratio being determined jointly by the inoculum and the host.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1964
- Accession Number
- AD0444379
Entities
People
- Fred P. Heydrick
- Lemoin B. Cree
- Ralph P. Wachter
Organizations
- United States Army Biological Warfare Laboratories