RICKETTSIAL GROWTH IN SUSPENSION CELL CULTURES IN SERUM-FREE AND DEFINED MEDIA
Abstract
Rickettsia typhi and Rickettsia rickettsii were propagated in cultures of cat kidney and L cell lines that were grown in a serum-free suspension system. One growth medium consisted of lactalbumin hydrolyzate and glutamine as nitrogen sources, glucose and pyruvate as carbon sources, vitamins, salts, and additives such as Methocel, insulin, and phenol red. A second and third medium consisted of essential amino acids with or without nonessential amino acids, respectively as nitrogen sources, salts, carbon sources, vitamins, and the additives, Methocel, insulin and phenol red. LAH supplemented with 10 per cent calf serum was used as a control. The two cell lines were carried as stock cultures in serum-free suspensions at least a month prior to the experiments. The studies were conducted with 100-ml serum bottles (30 ml medium per bottle) and were agitated on a New Brunswick shaker at 130 rpm at 35 C.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1964
- Accession Number
- AD0448485
Entities
People
- Henry J. Hearn Jr.
- Henry R. Tribble Jr.
- Horace B. Rees Jr.
Organizations
- United States Army Biological Warfare Laboratories