AMINO ACID COMPOSITION AND TERMINAL AMINO ACIDS OF STAPHYLOCOCCAL ENTEROTOXIN B
Abstract
The amino acid composition of purified staphylococcal enterotoxin B was determined by means of an automatic amino acid analyzer. All of the naturally occurring amino acids were found with no indication of any unusual amino acids. Extraordinarily high values of aspartic acid, glutamic acid and lysine were found, but a high amide nitrogen accounted for the alkaline isoelectric point. Isoionic protein with a pH of 8.55 was prepared by passing the toxin through a mixed bed ion exchange column. Glutamic acid was identified as the N-terminal amino acid through the fluorodinitrobenzene procedure. The Edman procedure confirmed this and showed that the residue was present in the protein as the free acid and not as glutamine. The C-terminal amino acid was found by means of hydrazinolysis to be lysine. Quantitative estimates showed 1. 10 residue. All results thus far obtained are consistent with the representation of the toxin as a single polypeptide chain.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1964
- Accession Number
- AD0449728
Entities
People
- David Stefanye
- Edward J. Schantz
- Henry M. Jacoby
- Leonard Spero
- Peter I. Brecher
Organizations
- United States Army Biological Warfare Laboratories