CHANGES IN CONCENTRATION OF BOTULINUM TOXIN IN DOG SERUM AFTER PARENTERAL ADMINISTRATION
Abstract
This experiment was planned to determine the amount of free botulinum toxin, Type A, in blood of dogs at various times after parenteral administration. The toxin was administered to the test subjects in doses of 8, 000 and 10,000 mouse intraperitoneal LD50 /kg by the intravenous, intraperitoneal, and intramuscular routes. Blood samples were taken at various times after injection, and the concentration of toxin in the serum was determined by a bioassay in mice using the mortality fraction or the time of death. The percent of injected dose found in the serum was plotted against sampling time to show the extent of transfer of toxin into the blood stream from the intraperitoneal and the intramuscular routes and its disappearance from the blood. Botulinum toxin administered intravenously, intraperitoneally, and intramuscularly in doses of 10,00 MU/kg to dogs can be detected in the serum by bioassay in mice as long as 2 to 4 days after injection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1964
- Accession Number
- AD0451095
Entities
People
- Fred W. Oberst
- James W. Crook
- Michael J. House
- Patl Cresthull
Organizations
- Edgewood Chemical Biological Center