A NEW METHOD FOR PURIFYING COXIELLA BURNETII BY DEXTRAN SULFATE PRECIPITATION
Abstract
This paper describes a simple method for purifying Coxiella burnetii cultivated in chick embryos. The procedure is based upon the preliminary clarification of infected whole-egg suspensions by precipitating the low-density lipoproteins and other extraneous solids using dextran sulfate, calcium chloride, and sodium chloride solutions, then concentrating the rickettsiae by centrifugation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1964
- Accession Number
- AD0452977
Entities
People
- Jack L. Davis
- William C. Patrick Iii.
Organizations
- United States Army Biological Warfare Laboratories