GROWTH CHARACTERISTICS OF PSITTACOSIS GROUP AGENTS IN HUMAN DIPLOID CELLS
Abstract
A comparison was made of the growth and morphological development of two agents of human pneumonitis (strains Borg and SF) and one agent of psittacosis (strain 6BC) in one cell line (L cells) and several commercially available HDCS, including lung, skin and muscle, kidney, and whole embryo strains. Growth of the three agents was supported by each of the diploid cell strains in all combinations that were tested, although the three agents were not tested in each strain. Results obtained by growing the agents in HDCS were essentially the same as those obtained in the L cell line. A cytopathic effect (CPE) described as floating cellular debris, rounding of cells, holes, and a granular appearance throughout parts of the cell monolayer was observed in all cell cultures and with each of the agents used. The two strains (SF and Borg) of the human pneumonitis agent differed in growth pattern, CPE observed in cell cultures, and the type of inclusion bodies formed in human lung cells. The SF agent resembled more closely the 6BC strain of the psittacosis agent. Comparative titers, cytopathology, and morphological development of the agents in HDCS are discussed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1964
- Accession Number
- AD0453397
Entities
People
- James T. Duff
- John W. Pearson
- Mary L. Robbins
- Naomi F. Gearinger
Organizations
- United States Army Biological Warfare Laboratories