RELATIONSHIP BETWEEN COMPOUND STRUCTURE AND INFECTIVITY EFFECT ON EEE VIRUS

Abstract

A number of sulfhydryl (-SH) compounds and the nonsulfur analogs of some of these compounds were tested for their effect on the infectivity of partially purified eastern equine encephalitis virus during storage at 4 C. Thiourea was found to be the most effective stabilizer; whereas urea and guanidine caused a loss of viral infectivity. Thiosemicarbazide and semicarbazide showed effects similar to those of thiourea and urea. Viral inactivation produced by p- chloromercuribenzoate, sodium thioglycollate, or sodium ascorbate could be prevented by thiourea. No correlation was found between the oxidation-reduction potential of virus suspensions containing the various compounds and the infectivity titer, or change in titer, of these suspensions. The presence of an -SH group in the molecule is not a requisite for stabilization: glutamine and dipyridyl exhibited good stabilizing properties.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1964
Accession Number
AD0453399

Entities

People

  • Joann F. Comer
  • Ralph F. Wachter

Tags

DTIC Thesaurus Topics

  • Acids
  • Antiviral Agents
  • Arbovirus Infections
  • Biological Laboratories
  • Cysteine
  • Encephalitis
  • Equine Encephalitis
  • Government Procurement
  • Nucleic Acids
  • Oxidation
  • Proteins
  • Reducing Agents
  • Rocket Oxidizers
  • Sulfur Compounds
  • United States
  • Viruses
  • Vitamin C

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Molecular and Cellular Biochemistry
  • Virology (or Medical Virology).