INTERACTION BETWEEN VEE VIRUS AND RICETTSIA RICKETTSII IN SUSPENDED L CELLS
Abstract
Rickettsia rickettsii were found to interfere with the growth of Venezuelan equine encephalitis virus in suspended L cell cultures grown in a defined medium. The effectiveness of the inhibition of viral growth was most pronounced in cultures in which the rickettsiae were introduced 2 days prior to the virus. Under these conditions, virus exhibited only minimal signs of growth. In cultures infected simultaneously the virus achieved its maximal titer but underwent an unusually rapid cessation of growth soon afterwards. The viral titer of L cell cultures chronically infected with VEE virus for 86 days disappeared 4 to 5 days after the inoculation of rickettsiae. Possible differences in susceptibility to virus and rickettsiae among the cells in culture were implicated as playing a role in this phenomenon. In support of this, virus multiplied in cat kidney cells to higher titers and rickettsiae to lower titers than in L cells. In the cat kidney cells, the rickettsiae failed to inhibit the growth of virus. The failure of virus to grow in 2-day-old rickettsial-infected L cell cultures, however, suggests that under the proper conditions the latter organism was capable of influencing the entire culture against viral growth.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1964
- Accession Number
- AD0455885
Entities
People
- Henry J. Hearn Jr.
- Henry R. Tribble Jr.
- Horace B. Rees Jr.
Organizations
- United States Army Biological Warfare Laboratories